Abstract:
The goal of the current study was to describe the cecum and colon's histological architecture and
histochemical characteristics in cape hares at various ages. From 15 healthy cape hare, samples of the
cecum and colon were taken: five at one day old, five at fourteen days old, and five at adulthood, Ten
samples from each area were collected and fixed in 10% formalin for 24 hours before being subjected
to standard histological procedures and having the sections stained with H&E, PAS, AB, and Masson's
Trichrome to identify the various components. At one day old, simple columnar epithelium
lined the mucosa of the cecum and colon, with fewer goblet cells and vacuolated cells, and no
crypts. The cecum's wall was thin. A lengthy spiral fold that extended into the ampulla caecoli
substantially expanded the interior surface of it. The appendix, which made up the remainder of the
cecum, had a thick wall and was extensively covered in lymph aggregates. At 14 days old, disappeared the
vacuolated cells and the villi developed into their mature form and the wall thickness had increased,
revealing the early crypts and an accumulation of lymphocytes in the lamina propria. The crypts were
fully grown and had thickened all of the structural levels of the wall at ages 40 day as an adult. Both the
proximal colon's lamina propria and submucosa were found to have highly developed lymphoid tissue,
The four tunicae made up the cecum and colon wall (mucosa, submucosa, muscularis and serosa or
adventitia). The muscularis mucosa, lamina propria with glands, and epithelium form tunica mucosa,
which was the inner layer bordering the lumen, were all visible. These tunica's average thickness in
the colon was higher than in the cecum. Large amounts of Lieberkuhn crypts and goblet cells were in
the colon and more of them than in the cecum. The submucosa is made up of many dense
connective tissues. These tunica's average thickness in the cecum was greater than that in the colon.
The tunica muscularis in the colon was thicker on average than in the cecum and was made up of
circular and longitudinal smooth muscle layers. The loose connective tissues that make up the tunica
serosa or adventitia were thicker on average in the colon than in the cecum. At one and 14 days ages,
the goblet cells reacted moderately with PAS, while strongly with AB. In adult age, the goblet cells
were moderate reaction with PAS and AB. |
